Explore topic-wise InterviewSolutions in Current Affairs.

This section includes 7 InterviewSolutions, each offering curated multiple-choice questions to sharpen your Current Affairs knowledge and support exam preparation. Choose a topic below to get started.

1.

What is polyembryony and how can it be commercially exploited?

Answer»

As in many Citrus and Mango varieties some of the nucellar cells surrounding the embryo sac start dividing, protrude into the embryo sac and develop into the embryos. In such species each ovule contains many embryos. Occurrence of more than one embryo in a seed is referred as polyembryony. If hybrids are made into apomicts, there is no segregation of characters in the hybrid progeny. Then the farmers can keep on using the hybrid seeds to raise new crop year after year and he does not have to buy hybrid seeds every year.

2.

Why does the zygote begin to divide only after the division of Primary Endosperm Cell (PEC)?

Answer»

The zygote needs nourishment during its development. As the mature, fertilised embryo sac offers very little nourishment to the zygote, the PEC divides and generates the endosperm tissue which nourishes the zygote. Hence, the zygote always divides after division of PEC.

3.

Are parthenocarpy and apomixis different phenomena? Discuss their benefits.

Answer»

Yes, they are different. Parthenocarpy leads to development of seedless fruits.
Apomixis leads to embryo development.

4.

Explain the following terms : False fruit,True fruit, Parthenocarpic fruit, Apomixis, Polyembryony,

Answer»

Thalamus become fruit, ovary becomes fruit, fruit formation without fertilisation, produce seeds without fertilization,more than one seed in a seed.

5.

Classify the following to form Column B as per the category given in Column A(i) Severo Ochoa (ii) F. Jacob and J. Monod (iii) Temin and Baltimore (iv) H. Winkler (v) T. H. Roderick (vi) R KornbergColumn AColumn B(1) Lac Operon————–(2) Central dogma in retroviruses————–(3) Coined the term Genome————–(4) Coined the term Genomics————–(5) Enzymatic synthesis of RNA————–(6) DNA is associated with histones and non-histones————–

Answer»
Column AColumn B
(1) Lac Operon(ii) E Jacob and J. Monod
(2) Central dogma in retroviruses(iii) Temin and Baltimore
(3) Coined the term Genome(iv) H. Winkler
(4) Coined the term Genomics(v) T. H. Roderick
(5) Enzymatic synthesis of RNA(i) Severo Ochoa
(6) DNA is associated with histones and non-histones(vi) R. Kornberg
6.

Pollination is prerequisite for fertilization in plants.

Answer»

1. Fertilization is fusion of male and female gametes. 

2. Pollination is transfer of pollen grains which carry non-motile male gametes. 

3. Pollen grains are transferred from anther to stigma of flower where they germinate. 

4. Both male and female gametes are nonmotile and they are produced at two different sites.

5. Therefore the pollination process is necessary for act of fertilization in plants.

7.

State what is apomixis. Comment on its significance. How can it be commercially used?

Answer»

Apomixis is a type of asexual reproduction that mimics sexual reproduction to form seeds without fertilisation. 

In apomictic seeds, parental characters are maintained in the progeny/offspring as there is no meiosis or segregation of characters. 

If desired hybrid seeds are made apomictics the farmers can keep on using the hybrid seeds to raise new crops year after year. 

8.

Complete the following table :Types of pollinationAgentKind of flowersExamples1. Anemophily.......Small, colourless,without,nectarMaize2......Bats............Adonsonia3. Entomophily.....Large, showy, Sweet with nectar.....4.BirdsBrightly coloured, large, showey with dilute nectar......

Answer»
Types of pollinationAgentKind of flowersExamples
1. Anemophilywind.Small, colourless,without,nectarMaize
2.ChiropterophilyBatsDull coloured, with stronge fragnance, abundantAdonsonia
3. EntomophilyInsectLarge, showy, Sweet with nectarrose/Jasmine
4.OrnithophilyBirdsBrightly coloured, large, showey with dilute nectarBombax/Butes
9.

Is pollination and fertilisation necessary in apomixis? Give reasons.

Answer»

No, they are not necessary. Apomixis is actually an alternative to sexual reproduction, although the female sexual apparatus is used in the process. In apomicts, embryos can develop directly from the nucellus or synergid or egg. Therefore, there is no need for either pollination or fertilisation.

10.

Are pollination and fertilization necessary in apomixis?

Answer»

Apomixis is formation of embryos without formation of gametes hence there is no need of pollination and fertilization.

11.

Mention the function of non-histone protein.

Answer»

Packaging of chromatin

12.

Write the role of histone protein in packaging of DNA in eukaryotes.

Answer»

Histones are positively charged basic proteins. The negatively charged DNA is wrapped around the positively charged histone octamer to form nucleosome.

13.

How many base pairs would a DNA segment of length 1.36 mm have?

Answer»

Distance between two base pairs = 0.34 mm or 0.34×10–6 mm Number of base pairs in 1.36 mm DNA segment

\(\frac{1}{0.34\times10^6}\) x 1.36

= 4 x 106 bp

14.

In prokaryotes ……………….. recognizes the promoter sequence. (a) alpha factor (b) rho factor (c) theta factor (d) sigma factor

Answer»

Correct answer is (d) sigma factor

15.

Match the following:ABPollenMonocarpellaryMonoeciousDiploidEndospemEmbryosacFemale gametophyteHaploidMale and female flower on same plantTriple Fusion

Answer»

Match the following:

AB
PollenHaploid
MonoeciousMale and female flower on same plant
EndospemTriple Fusion
Female gametophyteEmbryosac
16.

Filiform apparatus of an embryosac is located at(a) wall of embryosac(b) chalazal region(c) micropylar region (d) middle of polar nuclei (e) none of the above

Answer»

(c) micropylar region

17.

Can an unfertilized, apomictic embryosac give rise to a diploid embryo? If yes how?

Answer»

Yes, if embryo develops from the cells of nucellus or integuments, it will be diploid.

18.

Explain pollination.

Answer»

It is the transfer of pollen grains to the stigma of a pistil.

19.

Which of the following is genotypic ratio of Mendel’s monohybrid cross?(A) 1 : 3(B) 3 : 1(C) 1 : 2 : 1(D) 1 : 1 : 1 : 1

Answer»

(C) 1 : 2 : 1

1 : 2 : 1; one homozygous dominant, two heterozygous dominant and one homozygous recessive.

20.

The base sequence of one strand of DNA is TACTAGGAT.

Answer»

i. Write the base sequence of the RNA got after transcription of the given sequence. 

ii. What is the distance maintained between the two consecutive pairs of bases in the DNA molecule? 

iii. Who contributed the base complementary rule?

21.

If the base sequence of one strand of DNA is CAT TAG TAC GAC, what will be the base sequence(a) Of complementary DNA strand, and(b) Of its complementary RNA strand?

Answer»

(a) GTA ATC ATG CTG
(b) GUA AUCAUGCUG.

22.

The base sequence on one of the strands of DNA is ATGTCTATA. i. Give the base sequence of its complementary strand. ii. If an RNA strand is transcribed by this strand, what would be the base sequence of RNA ? iii. In what other respect, an RNA molecule differs from a DNA molecule ?

Answer»

(i) TACAGATAT 

(ii) UACAGAUAU

(iii)

S. No.DNARNA
(a)It is a double-stranded structure.It is a single-stranded structure.
(b)It contains deoxyribose sugarIt contains ribose sugar
(c)Bases are A, T, G, CBases are A, G, C, U

23.

As the base sequence present on one strand of DNA decides the base sequence of other 8 strand, this strand is considered as ……………….. (a) descending strand (b) leading strand (c) lagging strand (d) complementary strand

Answer»

Correct answer is (d) complementary strand

24.

After syngamy and triple fusion in embryosac, embryo will be diploid and endosperm will be .....

Answer»

After syngamy and triple fusion in embryosac, embryo will be diploid and endosperm will be Triploid.

25.

Mention the polarity of DNA strands a-b & c-d shown in the replicating fork given below :

Answer»

Polarity of DNA strand a-b is 3'--> 5' & c-d has polarity 5'--> 3'.

26.

In which phase of the cell cycle does replication occur in Eukaryotes ? What would happen if cell - division is not followed after DNA replication ?

Answer»

S-phase. 

Polyploidy

Detailed answer:

DNA replication takes place in S-phase (Synthetic phase) of cell cycle in eukaryotes. If this replication of DNA is not followed by cell division the DNA contents of the cell will get doubled i.e., duplicate set of DNA will be formed. This will result in autopolyploidy. 

27.

The monohybrid ratio is defined as(A) phenotypic ratio obtained in F2 generation of monohybrid cross.(B) phenotypic ratio obtained in F1 generation of monohybrid cross.(C) genotypic ratio obtained in F2 generation of monohybrid cross.(D) genotypic ratio obtained in F1 generation of monohybrid cross.

Answer»

Correct answer is

(A) phenotypic ratio obtained in F2 generation of monohybrid cross.

28.

Which of the following is phenotypic ratio of Mendel’s monohybrid cross ?(A) 1:2:1(B) 3:1(C) 1:1:2(D) 1:3

Answer»

Correct answer is

(B) 3:1

29.

The base sequence on one strand of DNA is ATGTCTATA i) Give the base sequence of its complementary strand. ii) If an RNA strand is transcribed from this strand what would be the base sequence of RNA? iii) What holds these base pairs together?

Answer»

i) TACAGATAT 

ii) UACAGAUAU 

iii) Hydrogen bonds

30.

Name the types of synthesis 'a' and 'b' occurring in the replication fork of DNA as shown below:

Answer»

a- Leading strand (continuous) 

b-Lagging strand (discontinuous).

31.

Why do you see two different types of replicating strands in the given DNA replication fork? Explain. Name these strands.

Answer»

The DNA-dependent DNA polymerase catalyses polymerisation only in one direction i.e., 5′→3′. Therefore, in one strand with polarity 3′→5′ continuous replication takes place whereas the other strand with polarity 5′→3′ carries out discontinuous replication. 

The strand with polarity 3′→5′ is called leading strand and the strand with polarity 5′→3′ is called lagging strand.

32.

Explain the process of DNA replication with the help of a replicating fork.

Answer»

There is a definite region in DNA where the replication originates called as origin of replication, For long DNA molecules since the two strands of DNA cannot be separated in its entire length, the replication occurs within a small opening of the DNA helix, referred to as replication fork. The DNA dependent DNA polymerase, catalyze polymerization only in one direction, that is 5' ---> 3', on one strand (the template with polarity 3' ---> 5') the replication is continuous, while on the other (the template with polarity 5' ---> 3;) it is discontinuous. The discontinuous synthesized fragments are later joined by the enzyme DNA ligase.

33.

Give two reasons why both the strands of DNA are not copied during DNA transcription?

Answer»

a) If both the strands act as template, they would code for RNA molecule with different sequences, and in turn they code for different proteins. 

b) the two RNA molecules if produced simultaneously would be complementary to each other , hence would for a double stranded RNA.

34.

Why do you see two different types of replicating strands in the given DNA replication fork ? Explain. Name these strands.

Answer»

(i) In long DNA molecules, since the two strands of DNA cannot be separated in its entire length, the replication occur within a small opening to the DNA helix, referred to as a replication fork. 

(ii) The DNA-dependent DNA polymerases catalyse polymerization of the nucleotides only in 5' ---> 3' direction. 

Consequently, on one of the template strands (with 3' --> 5' polarity) the synthesis of DNA is continuous, . while on the other template strand (with polarity 5' ---> 3'), the synthesis of DNA is discontinuous, i.e., short stretches of DNA are synthesized. 

(iii) The discontinuously synthesized strands are later joined together by the enzyme DNA ligase.

35.

DNA polymerase plays a dual function during DNA replication‖ comment on statement?

Answer»

It not only catalyses the polymerization reaction fast but also catalyses the reaction accurately.

36.

(a) Identify strands 'A' and 'B' in the diagram of transcription unit given above and write the basis on which you identified them. (b) State the functions of Sigma factor and Rho factor in the transcription process in a bacterium. (c) Write the functions of RNA polymerase-I and RNA polymerase-III in eukaryotes.

Answer»

(a) A-Template strand = 1 

B-Coding strand: 1 

Template strand has polarity 3'-5' 

Coding strand has polarity 5'-3' 

On the basis of polarity with respect to promoter.

(b) In initiation sigma factor associates with RNA polymerase to initiate transcription, Rho factor gets associated to RNA polymerase to terminate transcription.

(c) RNA polymerase I - Transcribes -RNAs 

RNA polymerase III - Transcribes tRNA / 5srRNA / 5nRNA.

37.

Name the three major types of RNAs, specifying the function of each in the synthesis of Polypeptide.

Answer»

(i) mRNA-(Messenger RNA) : decides the sequence of amino acids. 

(ii) tRNA-(Transfer RNA) : 

(a) Recognises the codon on mRNA 

(b) transport the aminoacid to the site of protein synthesis. 

(iii) rRNA (Ribosomal RNA) : Plays the structural and catalytic role during translation.

38.

Differentiate between continuous and discontinuous synthesis of DNA.

Answer»
ContinuousDiscontinuous
(a) One stand of DNA is synthesised as a continuous strech in the
5′ —>3′ direction.
(a) Short stretches  are synthesised in the 5’—>3′ direction from replication fork
(b) The template strand of DNA strand is with 3’—>5′ polarity.(b) The DNA strand with 5′ —> 3′ polarity is the template strand  for this.
(c) No need of enzyme ligase (for joining)(c) DNA ligase  enzyme is required  for joining short stretches.
(d) There is no need for primers.(d) There is need for primers.
39.

Define genetic code and write its salient features.

Answer»

Genetic code is the relationship between the sequence of nucleotides on mRNA and sequence of amino acids in the polypeptide.
Its features are

  • The genetic code is universal ie., the codons code for one amino acid is same in all organism.
  • Codons are triplet codons and there are 64 codons. 61 codons code for twenty different amino acids while the other three (UAA, UAG, UGA) are termination codons which do not code for any amino acids.
  • Each codon codes for only one particular amino acid. Therefore it is unambiguous.
  • Some amino acids are coded by more than one codon there for it is said to be degenerate.
  • The codons are read in a continuous manner, without any punctuation ie., codons are comma less.
  • AUG has dual functions of coding for metheonine as well as functioning as initiation codon.
40.

Which one out of Rho factor and Sigma factor acts as the initiation factor during transcription in a prokaryote?

Answer»

Sigma factor.

41.

For evolution to occur in a population, which of the following must happen? (A) The frequencies of some alleles in an organism's genotypes must change during its lifetime. (B) The frequencies of each allele in an organism's genotype must remain constant within its lifetime. (C) The frequencies of some alleles in a population's gene pool must change over successive generations. (D) The frequencies of each allele in a population's gene pool must remain constant over

Answer»

Correct option (C) The frequencies of some alleles in a population's gene pool must change over successive generations.   

Explanation:

Evolution may be described as the change in heritable characteristics of a population over successive generation. So, for evolution to occur, allele frequency must change for whole group of population over successive generation.

42.

Write short notes on different types of RNAs.

Answer»

The RNAs are of 3 types

  • Messenger RNA (m – RNA)
  • Transfer RNA (t – RNA)
  • Ribosomal RNA (r – RNA)

m – RNA It provides the template for polypeptide synthesis, ie., it decides the sequence of amino acids in the polypeptide through the sequence of bases on it. t – RNA It has the shape of clover leaf in two dimensional structure It transports the amino acids to the site of protein synthesis it recognises the codon on m RNA. r – RNA It plays the structural and catalytic role during translation.

43.

i. Describe the role of RNA polymerases in transcription in bacteria and in eukaryotes. ii. Name the scientist who postulated the role of an ‘adaptor’ in protein synthesis. Name the adaptor molecule.

Answer»

i. Role of polymerase in transcription in bacteria 

a. Initiation: σ (sigma) factor recognises the start signal and promotor region on DNA and the σ (sigma) with RNA polymerase binds to the promoter and initiates transcription. 

b. Elongation: The RNA polymerase after initiation of RNA transcription loses the σ-factor but continues the process of RNA formation. 

c. Termination: Once the RNA polymerase reaches the termination region of DNA, the RNA polymerase is separated from DNA-RNA hybrid, as a result nascent RNA separates. This process is called termination which is facilitated by certain termination factor . 

Role of polymerase in transcription in eukaryotes 

a. In eukaryotes, apart from the RNA polymerase found in the organelles, three types of RNA polymerases are found in the nucleus. 

b. RNA polymerase II transcribes the precursor of mRNA (called as heterogeneous nuclear RNA (hnRNA). 

c. RNA polymerase III helps in transcription of tRNA, 5S rRNA, and snRNAs (small nuclear RNAs). 

ii. Francis Crick postulated the role of an adaptor. tRNA or transfer RNA is the adaptor molecule.

44.

 Select the option that shows correct relationship between these organisms.Direction: Read the given passage and answer the following questions. Siddhi studied forelimbs of three organisms and described them as follows : Organism X - Long and short bones, covered with muscles, used for running. Organism Y - Long and short bones, covered with feathers used for flying. Organism Z - A layer of a complex chemical with supporting framework which is not made of bones and used for flying

Answer»

The Correct option is (A)

45.

What are the functions of three types of RNAs in bacteria? Which enzyme is involved in transcription of DNA to form RNAs in bacteria? What is its function?

Answer»

1. In bacteria, m-RNA provides the encoded message for protein synthesis; t-RNA brings specific amino acid to the site of translation; r-RNA plays role in providing binding site to m-RNA and tRNA.

2. There is single DNA dependent-RNA polymerase that catalyses transcription of all 3 types of RNA in bacteria. 

3. RNA polymerase binds to promoter and initiates transcription (initiation) and synthesizes RNA.

46.

With regard to mature mRNA in eukaryotes:a. exons and introns do not appear in the mature RNAb. exons appear but introns do not appear in the mature RNAc. introns appear but exons do not appear in the mature RNAd. both exons and introns appear in the mature RNA

Answer» b. exons appear but introns do not appear in the mature RNA
47.

Explain the elongation process of transcription in bacteria?

Answer»

Elongation process of Transcription in bacteria

-After binding to the promoter, the RNA polymerase facilitates the opening of the DNA.-It uses nucleoside triphosphates as substrate and polymerises the nucleotides in a templatdependent fashion following complementarity of bases in 5' → 3 direction The process continues till the RNA polymerase reaches the terminator region on the DNA stran.

48.

Monocistronic structural genes in eukaryotes have interrupted coding sequences, Explain. How are they different in prokaryotes?

Answer»

In eukaryotes, the hnRNA (primary transcript of mRNA) has coding sequences, 2 called exons as well as non-coding sequences, called introns, i.e., the information is split- If undergoes a process, called splicing, in which the introns are remove and the exons are joined together in a particular manner, to form the functional mRNA.- In prokaryotes, the mRNA is polycistronic, i.e., codes for more than one polypeptide.- The information is continuous and no splicing is required.

49.

If there are two pathogenic viruses, one with DNA and other with RNA, which would mutate faster? And why?

Answer»

Both DNA and RNA are able to mutate. In fact, RNA being unstable, mutate at a faster rate. Consequently, viruses having RNA genome and having shorter life span mutate and evolve faster.

50.

Autogamy can occur in a chasmogamous flower if:a. Pollen matures before maturity of ovuleb. Ovules mature before maturity of pollenc. Both pollen and ovules mature simultaneouslyd. Both anther and stigma are of equal lengths.

Answer» c. Both pollen and ovules mature simultaneously