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Write a lab activity on stained lactobacillus bacterium |
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Answer» Requirements • MICROSCOPIC glass slides • Microscopic cover slips •Yogurt sample • Blotting paper • Cotton swabs • Methylene blue solutionStaining1)The staining process will also require the use of heat and methylene blue stainPlace a drop of dilute yogurt solution on the microscopic slide and spread it to form a thin smear2)Allow the slide to dry in air (without using heat) to form a thin film3)Quickly pass the slide three times above the flame (a bunsen burner can be used) to fix the bacteria. The side with film should be up4)Allow the slide to cool to room temperature5)Add a drop of methylene blue stain on the film and allow it to settle for about 2 minutes6)Gently rinse excess stain in a beaker of WATER7)Place a cover SLIP on the film and observe under the MICROSCOPE starting with LOW magnificationExplanation:ObservationThe two types of bacteria observed are;Lactobacillus bulgaricus (rod shaped)Streptococcus thermophilus (spherical |
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