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Which of the following plot is also known as a double reciprocal plot?(a) Line-weaver burk plot(b) Eadie-Hofstee plot(c) Michaelis-menten plot(d) Langmuir plot |
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Answer» Right answer is (a) Line-weaver burk plot For explanation I would say: The double-reciprocal equation is obtained by taking the reciprocal of both sides of the Michaelis-Menten equation. The double-reciprocal (also known as the Lineweaver-Burk) plot is created by plotting the inverse initial velocity (1/V0) as a function of the inverse of the substrate concentration (1/[S]). The vmax can be accurately determined and thus KM can also be determined with accuracy because a straight line is formed. The slope of the resulting line is KM/vmax, the y-intercept is 1/vmax, and the x-intercept is -1/KM. |
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