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Which of the following is true about Michaelis-Menten kinetics?(a) Km, the Michaelis constant, is defined as that concentration of substrate at which enzyme is working at maximum velocity(b) It describes single substrate enzymes(c) Km, the Michaelis constant is defined as the dissociation constant of the enzyme-substrate complex(d) It assumes covalent binding occurs between enzyme and substrate |
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Answer» The correct answer is (b) It describes single substrate enzymes To explain: Km is defined as the concentration of substrate at which enzyme is working at half of maximum velocity. It is also a measure of the affinity that the enzyme has for its substrate. Michaelis-Menten kinetics assumes non-covalent binding between enzyme and substrate. |
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