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What temperature is suitable for heat-shock treatment?(a) 100°C(b) 0°C(c) 42°C(d) 95°CI had been asked this question in an online interview.Origin of the question is Tools of Recombinant DNA Technology: Competent Host in portion Biotechnology Principles and Processes of Biology – Class 12

Answer»

Correct option is (c) 42°C

For explanation: 42°C-45°C is the ideal TEMPERATURE for heat-shock treatment. Mostly calcium chloride is USED in this. It is suitable for the TRANSFORMATION of BACTERIAL cells such as E.coli.



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