What is Tissue culture? Give the methods of Tissue culture.

1.	What is Tissue culture? Give the methods of Tissue culture.
Answer» Technique of in vitro regeneration of whole plant by growing any plant part called explant in culture medium under aseptic condition. Includes following methods: 1. Callus culture: Cell division in explant form an unorganized mass of cell called callus. 2. Suspension culture: Involves small group of cells suspended in a liquid media. 3. Meristem Culture – Apical shoot meristem is used as explant & support multiple shoot development. 4. Embryo Culture: Excision of young embryos from developing seeds & culture in nutritional media. 5. Anther culture: Production of haploid plant species by desired anther cultured in suitable medium.

Answer»

Technique of in vitro regeneration of whole plant by growing any plant part called explant in culture medium under aseptic condition.

Includes following methods:

1. Callus culture: Cell division in explant form an unorganized mass of cell called callus.

2. Suspension culture: Involves small group of cells suspended in a liquid media.

3. Meristem Culture – Apical shoot meristem is used as explant & support multiple shoot development.

4. Embryo Culture: Excision of young embryos from developing seeds & culture in nutritional media.

5. Anther culture: Production of haploid plant species by desired anther cultured in suitable medium.

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