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What is the mechanism of muroxide tests |
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Answer» The murexide test for uric acid consists in the reaction of uric acid with dilute nitric acid, followed by ammonia addition: a purple colouration is produced due to the formation of AMMONIUM purpurate or murexide. The reaction mixture is heated to dryness in a porcelain dish on the water bath.Explanation:The murexide test is an analytical technique to IDENTIFY the presence of caffeine and other purine derivatives in a sample. These compounds do not respond to the common alkaloid detecting tests such as Dragendorff's reagent. In this test the alkaloids are mixed with a tiny amount of potassium chlorate and a drop of hydrochloric acid. The sample is then EVAPORATED to dryness and the resulting residue is exposed to ammonia vapour. Purine alkaloids PRODUCE a PINK colour in this test. Murexide (ammonium purpurate) with a purple colour is also produced in this test |
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