Saved Bookmarks
| 1. |
What is the major role of ice cold calcium chloride in the preparation of competent cells? |
|
Answer» The process of calcium chloride heat-shock transformation encourages bacterial cells to uptake DNA from the surrounding environment.The ice-cold CaCl2 solution facilitates binding of DNA to the surface of the cell, which then enters the cell after a short period of heat- shock (3). |
|