The restriction endonuclease EcoR-I recognizes and cleaves DNA sequenc

1.	The restriction endonuclease EcoR-I recognizes and cleaves DNA sequence as shown below 5' -G A A T T C-3' 3' -C T T A A G-5' What is the probable number of cleavage sites that can occur in a 10 kb long random DNA sequence ?(A) 10 (B) 2 (C) 100 (D) 50
Answer» Correct Option :- (B) 2 Explanation :- Eco RI is an example of six-cutter restriction endonuclease. It usually cleaves once in every 4096 bp. (1/4)⁶ = 1/4096 Given length of DNA fragment= 10 Kb = 10000 bp Hence, Probable no. of cleaving sites = 10000/4096 = 2.44

The restriction endonuclease EcoR-I recognizes and cleaves DNA sequence as shown below 5' -G A A T T C-3' 3' -C T T A A G-5' What is the probable number of cleavage sites that can occur in a 10 kb long random DNA sequence ?(A) 10 (B) 2 (C) 100 (D) 50

Answer»

Correct Option :- (B) 2

Explanation :-

Eco RI is an example of six-cutter restriction endonuclease. It usually cleaves once in every 4096 bp.

(1/4)⁶ = 1/4096

Given length of DNA fragment= 10 Kb = 10000 bp Hence,

Probable no. of cleaving sites = 10000/4096 = 2.44

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The restriction endonuclease EcoR-I recognizes and cleaves DNA sequence as shown below 5' -G A A T T C-3' 3' -C T T A A G-5' What is the probable number of cleavage sites that can occur in a 10 kb long random DNA sequence ?(A) 10 (B) 2 (C) 100 (D) 50

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