1.

Scientists have succeeded in recovering healthy sugarcane plants from a diseased one. (a) Name the part of the plant used as explant by the scientists. (b) Describe the procedure the scientists followed to recover the healthy Plants. (c) Name this technology used for crop improvement.

Answer»

(a) Meristem (apical, axillary) or shoot tip or nodal segment.

(b) Procedure, the scientists followed to recover healthy plants is :

Explant / Virus free meristem is grown in nutrient medium, under aseptic conditions, tissue proliferates to form undifferentiated mass called callus, transferred to a medium containing auxins and cytokinins, regeneration of plants from callus, Hardening of plant lets, transfer of regenerated plant lets to field conditions. 

(c) Tissue culture / micropropagation.



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