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Role of phenol chloroform dna extraction protocol from blood

Answer»

t of white cells should FORM at the bottom of the tube. ... Incubate over night at 37oC or at 55oC for 4-5 HOURS, after which the SOLUTION should be less VISCOUS, due to the white blood cells being broken down. Check that the sample has been lysed adequately before proceeding with the phenol-chloroform extraction.



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