Question : Assume that you are trying to insert a gene into a plasmid and someone gives you a preparation of DNA cut with restriction enzyme X. The gene tou wish to insert has sites on both ends for cutting by restriction enzyme Y. You have a plasmid with a single site for Y, but not for X. Your strategy should be to

Question : Assume that you are trying to insert a gene into a plasmid

1.	Question : Assume that you are trying to insert a gene into a plasmid and someone gives you a preparation of DNA cut with restriction enzyme X. The gene tou wish to insert has sites on both ends for cutting by restriction enzyme Y. You have a plasmid with a single site for Y, but not for X. Your strategy should be to
Answer» Cut the plasmid with RESTRICTION enzyme X and INERT the fragments cut with Y into the plasmid Cut the plasmid with restriction enzyme X and insert the gene into the plasmid Cut the plasmid TWICE with restriction enzyme Y and ligate the TWO fragments into the plasmid cut with the same enzyme Cut the plasmid twice with restriction enzyme Y and ligate the two fragments ONTO the ends of the human DNA fragments cut with restriction enzyme X Answer :C