Question : Assertion : The RNAi can be introduced in an organism by insertion of gene encoding complementary RNA only Reason : There are no methods by which in vitro synthesised complementary RNA can be inserted in an organism to induce RNAi (RNA interference)

Question : Assertion : The RNAi can be introduced in an organism by in

1.	Question : Assertion : The RNAi can be introduced in an organism by insertion of gene encoding complementary RNA only Reason : There are no methods by which in vitro synthesised complementary RNA can be inserted in an organism to induce RNAi (RNA interference)
Answer» If both assertion and reason are true and reason is the correct explanation of assertion If both assertion and reason are true but reason is not the correct explanation of assertion If assertion is true but reason is false If both assertion and reason are false Solution :RNAi (RNA interference) takes place in all enkaryotic organisms as a method of cellular DEFENSE. This method involves silencing of a specific mRNA DUE to a COMPLEMENTARY dsRNA molecule that binds to and prevents translation of the mRNA. There are two methods to induce RNA interference in any organism. The complementary RNA can be synthesisted in vitro and then annealed to PRODUCE dsRNA and insterted into cells or the cells are genetically modified to produce the two complementary RNA stands or duplex from in vivo by transcription. However, the first method i.e, INSERTION of vitro synthesised complementary RNA inhibits target gene expression for only 4-6 days and their re-introduction is required. But the second method i.e, the DNA vector construct once transfected into the cells will keep on generating RNA interference and thereby,cause a permanent inhibition of the damage.