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Principles of acid fast staining

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Acid-Fast Stain- PRINCIPLE, Procedure, Interpretation and EXAMPLES. It is the DIFFERENTIAL staining techniques which was first developed by Ziehl and LATER on modified by Neelsen. ... Neelsen in 1883 used Ziehl's carbol-fuchsin and heat then decolorized with an acidalcohol, and counter stained with methylene BLUE.



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