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Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool. |
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Answer» i. Restriction endonucleases: for cutting the desired DNA at desired places ii. Gel electrophoresis: for separating the desired DNA fragments iii. Ligase enzyme: for creating recombinant DNA molecule. iv. DNA delivery system: like electroporation, microinjection, gene gun method. v. Competant host (usually bacteria / yeast): to take up recombinant DNA |
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