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List the steps to prepare temporary mount of onion peel |
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Answer» 1. Take an onion and remove its OUTERMOST peel. 2. Now cut a small part from an inner scale leaf with the help of a blade. 3. Separate a thin, TRANSPARENT peel from the convex surface of the scale leaf with the help of forceps. 4. Keep this peel in a watch glass containing water 5. Add two drops of safranin stain in the watch glass to stain the peel. 6. Take a clean slide and put a drop of glycerine in the CENTRE of the slide. 7. With the help of a brush and needle transfer the peel on the slide. Glycerine prevents the peel from drying up. 8. CAREFULLY cover it with a coverslip and avoid any AIR bubble from entering interring the coverslip. 9. Remove any excessive glycerine with a filter paper. 10. Observe the prepared mount of the peel under the low and high magnification of a compound microscope. |
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