In the process of r-DNA technology, if two separate restriction enzymes are used to cut vector and donor DNA then which problem will arise in the formation of r-DNA or chimeric DNA? Explain.

In the process of r-DNA technology, if two separate restriction enzyme

1.	In the process of r-DNA technology, if two separate restriction enzymes are used to cut vector and donor DNA then which problem will arise in the formation of r-DNA or chimeric DNA? Explain.
Answer» In the process of r-DNA technology, if two separate restriction enzymes are used to cut vector and donor DNA, then it will result in fragments with different sticky ends which will not be complementary to each other.

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In the process of r-DNA technology, if two separate restriction enzymes are used to cut vector and donor DNA then which problem will arise in the formation of r-DNA or chimeric DNA? Explain.

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