In a paternity dispute, the VNTR DNA samples of parent and child were

1.	In a paternity dispute, the VNTR DNA samples of parent and child were DNA finger printed. The diagrammatic representation of the DNA fingerprint is shown below.(a) What is your opinion about the paternity of child? Substantiate your opinion.(b) List down any four major steps of molecular biological procedures adopted for this.
Answer» (a) Paternity is confirmed VNTR bands / finger print bands / bands are similar. (b) Steps of DNA finger printing techniques are (i) Isolation of DNA (ii) Digestion of DNA by restriction endo nuclease (iii) Seperation of DNA fragment by Electrophoresis, (iv) Plotting of DNA fragment into Nitro cellulose (v) Ditection of hybridised DNA by Auto radiography

In a paternity dispute, the VNTR DNA samples of parent and child were DNA finger printed. The diagrammatic representation of the DNA fingerprint is shown below.(a) What is your opinion about the paternity of child? Substantiate your opinion.(b) List down any four major steps of molecular biological procedures adopted for this.

Answer»

(a) Paternity is confirmed

VNTR bands / finger print bands / bands are similar.

(b) Steps of DNA finger printing techniques are

(i) Isolation of DNA

(ii) Digestion of DNA by restriction endo nuclease

(iii) Seperation of DNA fragment by Electrophoresis,

(iv) Plotting of DNA fragment into Nitro cellulose

(v) Ditection of hybridised DNA by Auto radiography