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Imagine you are generating a site-directed mutant of a protein in which a given residue is replaced by anotherresidue. Which one of the following substitutions will result in the highest isoelectric point of the mutantcompared to the wild type? (X → Y means residue X in the wild type is replaced by residue Y in the mutant).a. Asp → Argb. Arg → AspC. Tyr Lysd. Gly → Argplease explain how to solve. |
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Answer» Answer: a is the answerSite-directed mutagenesis of an amino ACID residue in the bacteriophage P2 OGR protein IMPLICATED in interaction with ESCHERICHIA coli RNA polymerase |
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