1.

How has recombinant technology helped in large scale production of vaccines? Explain giving one example.

Answer»

Production of insulin by rDNA techniques was achieved by an American company, Eli Lilly, in 1983. It prepared two DNA sequences corresponding to A and B chains of human insulin and introduced them in plasmids of E. coli for production. The A and B chains produced were separated, extracted and combined by creating disulfide bonds to form human insulin.



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