How did the chemical nature of the 'Transforming principle' get establ

1.	How did the chemical nature of the 'Transforming principle' get established ?
Answer» Purified biochemicals (protein, DNA, RNA) from heat killed S - strain. Treated with protease - did not affect transformation. Treated with RNase - did not affect transformation. Treated with DNase - transformation affected. Detailed answer: Chemical nature of transforming principle was established by Avery, Macleod and mccarty. They purified the biochemical (proteins, DNA, RNA etc) isolated from heat killed S-cells. They discovered (a) That digestion of protein and RNA, using the enzymes proteases and RNases did not affect transformation. So the transforming principle was not protein and RNA. (b) While digestion of DNA with DNase inhibited transformation. On this basis they concluded that DNA caused transformation of R-cells to S-cells, i.e., DNA was the transforming principle.

How did the chemical nature of the 'Transforming principle' get established ?

Answer»

Purified biochemicals (protein, DNA, RNA) from heat killed S - strain.

Treated with protease - did not affect transformation.

Treated with RNase - did not affect transformation.

Treated with DNase - transformation affected.

Detailed answer:

Chemical nature of transforming principle was established by Avery, Macleod and mccarty. They purified the biochemical (proteins, DNA, RNA etc) isolated from heat killed S-cells. They discovered (a) That digestion of protein and RNA, using the enzymes proteases and RNases did not affect transformation. So the transforming principle was not protein and RNA. (b) While digestion of DNA with DNase inhibited transformation. On this basis they concluded that DNA caused transformation of R-cells to S-cells, i.e., DNA was the transforming principle.

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