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Find the correct match for the breaking of the cell wall during isolation of genetic material in rDNA procedureA. Cellulase -Plant cellB. Lysozyme-FungusC. Chitinase-BacteriaD. All of the above |
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Answer» Correct Answer - A `**` In order to cut the DNA with restriction enzymes, it needs to be in pure form, free from other macromolecules `**` Since the DNA is enclosed within the membranes, we have to break the cell open to release DNA along with other macromolecules such as RNA, protein, polysaccharides and also lipids `**` This can be achieved by treating the bacterial cells/plant or animals tissue with enzymes such as: (i) Lysozyme (bacteria) (ii) Cellulase (plant cells) (iii) chitinase (fungus) |
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