1.

Explain the Dilution Factor calculation method

Answer»

Dilution Factor = final volume/initial volume.    

DF = Vf /V 

For Example :    

How would you make 500 mL of a 1:250 dilution?    

Solution:   

DF = Vf /Vi    

Vi = Vf /DF = 500mL/250 = 2.00 mL    

Pipet 2.00 mL of your stock solution into a 500 mL volumetric flask.    

Add diluent to the mark on the flask (you will have added about 498 mL of water). 

You now have a 1:250 dilution of your original solution.



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