Saved Bookmarks
| 1. |
Estimation of salivary nitric oxide and uric acid levels in oral squamous cell carcinoma and healthy controls |
|
Answer» imation of NO was done by standard kit (Agappe Diagnostics, India Ltd). NO CONCENTRATION is based on the enzymatic conversion of nitrate to NITRITE by colorimetric detection of nitrite as an AZO by-product of the Griess reaction. The Griess reaction is based on the two-step diazotization reaction in which acetified NO produces a nitrosating agent, which reacts with sulfanilic acid to produce diazonium ion. This ion is then COUPLED to N-ethylenediamine to form the chromophoric azo derivatives, which absorb light at 540-570 nm. The measurement of absorbance at 550 nm is read after 10 min. The concentration is calculated from a calibration PLOT prepared from a series of standard nitrite. |
|