1.

During agglutination reaction why is it important to wash the cells?

Answer»

Need to wash the cells: 

1. Washing improves reactivity. 

2. Removes plasma that contains fibrinogen and forms clot when mixed with serum giving false positive. 

3. Plasma can cause rouleaux’s formation. 

4. Anticoagulant present in plasma is anticomplementary and inhibits complement binding reactions. 

5. Plasma contains blood group substances that can neutralize that reaction.



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