Answer:

Meaning of Cell Suspension Culture:

Cell suspension culture is the culture of cells or cell aggregates DISPERSED in a moving liquid medium. Batcher and Ingram (’76) noted that all suspension cultures contain cell aggregates in addition to free cells

Cell suspension cultures were grown successfully in several plants, such as, Nico­tiana tabacum, Tagetes erecta, Daucus CAROTA, Antirrhinum and Picea glauca. Suspension cultures from various plants show different proportions of free cells and cell aggregates.

In suspension culture of Antirrhinum cell aggregates are more frequent than free cells. In suspension culture of Paul’s Scarlet rose free cells occur at a very high frequency.

Cells of a suspension culture often possess large nuclei, dense cytoplasm and starch grains. Cells show hormone habituation, increase in ploidy level and loss in totipotency. Cell separation is high and cell doubling period is 24-78 hours. But cell suspensions from different sources vary considerably in the expression of these characteristics.

Suspension Culture of Carrot

Suspension cultures are mainly of two types—batch culture and continuous culture.

(a) In batch culture cells are grown in a fixed volume of medium. It is a CLOSED system culture.

(b) In continuous culture by pumping a constant flow of the culture medium is maintained in the culture vessel. So there is REPLACEMENT of EQUAL volume of culture medium. New cell formation is more or less equal to the amount of old washed out cells. Thus the culture is maintained in a steady condition.

Continuous culture may be closed or open type. In an open system fresh medium is added and spent medium is withdrawn and the cells are also harvested. In a closed culture all cells remain in the culture, thus the number of cells increases until the stationary phase is readied.

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