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Differnces betwn gram positive n gram negative bacteria |
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Answer» Answer: Gram-negative Bacteria Gram-positive Bacteria Gram reaction Can be decolourized to accept counter stain (Safranin or Fuchsine); stain red or pink, they don't retain the Gram stain when WASHED with absolute alcohol and acetone. Retain crystal violet dye and stain dark violet or purple, they remain coloured BLUE or purple with gram stain when washed with absolute alcohol and water. PEPTIDOGLYCAN layer Thin (single-layered) Thick (multilayered) Teichoic ACIDS Absent Present in many Periplasmic space present Absent Outer membrane Present Absent Lipopolysaccharide (LPS) content High Virtually none Lipid and lipoprotein content High (due to presence of outer membrane) Low (acid-fast bacteria have lipids linked to peptidoglycan) Flagellar structure 4 rings in basal body 2 rings in basal body Toxins produced PRIMARILY Endotoxins Primarily Exotoxins |
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