1.

Difference between gram positive and gram negative stain.[in points]

Answer»

Characteristics Gram Positive Gram Negative

1. Gram Reaction Retain crystal violet DYE and stain blue or purple.

Gram-Positive

Accept safranin and stain pink or red

Gram-Negative

2. Cell Wall STRUCTURE Structure of Gram Positive cell wall :

gram-positive-cell-wall Structure of Gram Negative cell wall :

gram-negative-cell-wall

3. Cell Wall Thickness Thick (20-80 nm) Thin (8-10 nm)

4. Chemical Composition of Cell Wall Peptidoglycan, Teichoic acid Lipotechoic acid Lipopolysaccharide, Lipoproteins and Peptidoglycans

5. Peptidoglycan LAYER Thick (MULTILAYERED) Thin (Single LAYERED)

6. Teichoic Acid in Cell Wall Present Absent



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