1.

Difference between brightfield and darkfield microscopy

Answer»

Brightfield microscopy is the CONVENTIONAL technique. It is suitable for observing the NATURAL colors of a specimen or the observation of stained samples. The specimen APPEARS darker on a bright background.

Darkfield microscopy shows the specimens bright on a dark background. Brightfield microscopes that have a condenser with a filter holder can be easily converted to darkfield by placing a patch stop filter into the filter holder. The specimens appear bright, because they reflect the LIGHT from the microscope into the OBJECTIVE.

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