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Determination of protein and how much temp content by kjeldahl method

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\boxed{\sf\pink{Kjeldahl's\:method}}

✔ In this method, the compound

heated with concentrated sulphuric acid in the presence of SMALL amount of {CuSo_4}to convert nitrogen into Ammonium Sulphate

✔The contents of the flask are transferred to another flask and heated with excess of SODIUM hydroxide solution to liberate ammonia GAS

✔ Ammonia gas so liberated is passed and absorbed known as the volume concentrated sulphuric acid

✔Organic compound +{H_2SO_4{\rightarrow}(NH_4)_2SO_4}

{(NH_4)_2SO4+2NaOH{\rightarrow}Na_2SO_4+2H_2O+72NH_3}

{2NH_3+H_2SO_4{\rightarrow}(NH_4)_2SO_4}



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