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Can taq polymerase tailed pcr products be used directly for ligation |
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Answer» Explanation: If you are amplifying from a plasmid or simple template, there is very little chance for mis-priming, so you can use a PRETTY WIDE RANGE of ANNEALING temperatures, but you may NEED to increase your primer length and increase the Tm if you are trying to clone from genomic DNA, a cDNA library, or by RT-PCR |
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