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.Among different test methods, serological test is very popular. Explain what is ‘rRt-PCR’ and also state what respiratory samples does it work on? |
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Answer» Answer: Real-time reverse-transcription polymerase chain reaction (rRT-PCR) assays are MOLECULAR tests that can be used to detect viral RNA in clinical samples. CDC’s current case definition for laboratory CONFIRMATION of MERS-CoV infection requires either a positive rRT-PCR result for at least two specific genomic targets, or a single positive TARGET with sequencing of a second target. The success of rRT-PCR testing depends on SEVERAL factors, including the experience and expertise of laboratory personnel, laboratory environment (e.g., avoidance of contamination), and the type and condition of specimens being tested. For this rRT-PCR assay, CDC recommends COLLECTING multiple specimens, including lower (bronchalveolar lavage, sputum and tracheal aspirates) and upper (e.g., nasopharyngeal and oropharyngeal swabs) respiratory samples, serum, and stool specimens. patient to be negative for active MERS-CoV infection following two consecutive negative rRT-PCR tests on all specimens. |
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