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Agrobacterium tumifaciens is a natural vector. How?

Answer»

Agrobacterium Ti plasmid is preferred over all other vector because of wide host range of its bacterial system and the capacity to transfer genes due to presence of T-DNA border sequence. Agrobacterium tumefacieni said to be natural genetic engineer of plants due to the reason due to their capability to transfer T-DNA of their plasmid into desired plant genome at wound site. It leads of the formation of crown gall (tumour) i.e., unorganised growth of cells. Crown gall tumours synthesize, large amount of certain metabolites called opines. The genes which determine opine synthesis are borne on Ti plasmid.



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