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AIM ...

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to prepare a temporary mount of leaf peel to show its STOMATA ......

Requirements ...

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Leaf of a plant like lily, Bryophyllum , or tradenscantia, forceps, waych glass, slide, coverslip , brush, needles, safranin, glycerine, and microscope .

Principal Involved ...

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Stomata are minute appertures ( openings ) present on the surface of the leaf . they are generally more on the lower EPEDERMIS . Each stomata is surrounted by two bean-shaped cells called “gaurd cells” . Theae cells have differential thickenings, i.e. THICKER inner wall facing the stoma and thinner outer wall . The turgidity of gaurd cells controls the opening and closing of stomata . Stomata heps in exchange of gases and water vapours between the atmosphere land leaf .

Procedure ...

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1 ) Pell off the leaf from its under surface and put the peel in a watch glass containing water .

2 ) Add a few drops of Safranin stain into the watch glass to stain the peel .

3 ) After staining put the peel on the cleaning slide and cut it in a RECTANGULAR or square shape .

4 ) With the help of a filter paper, remove the excess of stain and water .

5 ) Put a drop of glycerine on the slide over the peel, gently put the coverslip to avoid the entry of air bubbles and observe under the microscope .

Observations ...

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1 ) Epidermis consists of a single layer of cells which are irregular in outline and without intracellular spaces .

2 ) Minute appertures ( openings ) are seen which are imbedded in epidermal cells .

Result ...

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The minute apperture seen in the mount are stomata . Each stomata is surrounded by two bean-shaped cells called gaurd cells .

Precautions ...

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1 ) The epidermal peel should be taken from a freshly plucked leaf .

2 ) Always hold the slide by its edges to avoid making the slide dirty .

3 ) Always use a brush to transfer the peel from petri dish to the slide .

4 ) The peel should be cut to a PROPER size and its curling must be avoided .

5 ) The peel should be mounted in the centre of the slide .

6 ) The peel should not be allowed to dry .

7 ) Always keep the coverslip gently to avoid the entry of air bubbles .

8 ) Oozing of glycerine should not be there .

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