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A sugarcane has been affected by the virus. How can a virus free cane developed from it? |
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Answer» Virus free sugarcane can be developed by tissue culture technique. The meristematlc regions of a plant are generally free from virus, Healthy and virus free sugarcane plants can by developed by this techmique using meristems as explants. Procedure : Explants obtained from the meristem apical or young axillary buds or from nodes near the stem apex are taken and surface sterlized by 0.5% Sodium hypochlorite and then seeded over the nutrient agar medium enriched with cytokinin under aseptic conditions. After incubation a few days under optimal conditions the explants microprogive rise to shoots. these shoots are cut into nodal Jegments of 2-3 cm which are then transferred into a medium containing extra auxin for promoting root formation. This results in the development of a number of plantlets, which are then hardened and transferred to fields. |
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