(a) Name the type of DNA that forms the basis of DNA fingerprinting an

1.	(a) Name the type of DNA that forms the basis of DNA fingerprinting and mention two features of this DNA. (b) Write the steps carried out in the process of DNA fingerprinting technique, and mention its application.
Answer» (a) Satellite DNA or repetitive DNA These sequences normally do not code for any proteins. These sequence show high degree of polymorphism. (b) (i) isolation of DNA, (ii) digestion of DNA by restriction endonucleases, (iii) separation of DNA fragments by electrophoresis, (iv) transferring (blotting) of separated DNA fragments to synthetic membranes such as nitrocellulose or nylon, (v) hybridization on using labelled VNTR probe, (vi) detection of hybridized DNA fragments by autoradiography The applications of DNA fingerprinting technique are in Forensic science / determining population and genetic diversities / paternity test.

(a) Name the type of DNA that forms the basis of DNA fingerprinting and mention two features of this DNA. (b) Write the steps carried out in the process of DNA fingerprinting technique, and mention its application.

Answer»

(a) Satellite DNA or repetitive DNA These sequences normally do not code for any proteins. These sequence show high degree of polymorphism.

(b) (i) isolation of DNA,

(ii) digestion of DNA by restriction endonucleases,

(iii) separation of DNA fragments by electrophoresis,

(iv) transferring (blotting) of separated DNA fragments to synthetic membranes such as nitrocellulose or nylon, (v) hybridization on using labelled VNTR probe, (vi) detection of hybridized DNA fragments by autoradiography The applications of DNA fingerprinting technique are in Forensic science / determining population and genetic diversities / paternity test.

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